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mouse monoclonal anti-flag antibody m20008  (Abmart Inc)

 
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    Abmart Inc mouse monoclonal anti-flag antibody m20008
    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal <t>anti-FLAG</t> antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse <t>monoclonal</t> anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).
    Mouse Monoclonal Anti Flag Antibody M20008, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti-flag antibody m20008/product/Abmart Inc
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti-flag antibody m20008 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis"

    Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2024.1533640

    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal anti-FLAG antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse monoclonal anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).
    Figure Legend Snippet: Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal anti-FLAG antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse monoclonal anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).

    Techniques Used: Co-Immunoprecipitation Assay, Stable Transfection, Expressing, Immunoprecipitation, Modification



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    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal <t>anti-FLAG</t> antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse <t>monoclonal</t> anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).
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    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal <t>anti-FLAG</t> antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse <t>monoclonal</t> anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).
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    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal <t>anti-FLAG</t> antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse <t>monoclonal</t> anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).
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    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal <t>anti-FLAG</t> antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse <t>monoclonal</t> anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).
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    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal <t>anti-FLAG</t> antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse <t>monoclonal</t> anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).
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    Image Search Results


    Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal anti-FLAG antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse monoclonal anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).

    Journal: Frontiers in Microbiology

    Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

    doi: 10.3389/fmicb.2024.1533640

    Figure Lengend Snippet: Co-immunoprecipitation assay for the interaction between AFAP and NPM1, and AFAP and DDB1. The cell lysates (input) from HEK293 cells stably expressing AFAP-SF were subjected to immunoprecipitation (IP) with rabbit polyclonal anti-HA antibody (mock), rabbit polyclonal anti-nucleophosmin antibody ( α -NPM1) (A) , rabbit polyclonal anti-FLAG antibody (α-FLAG) (B,D) , and rabbit polyclonal anti-DDB1 antibody (α-DDB1) (C) . Immunoprecipitates were immunoblotted (IB) with mouse monoclonal anti-nucleophosmin (α-NPM1) antibody, mouse monoclonal anti-FLAG antibody (α-FLAG), or rabbit polyclonal anti-DDB1 antibody (α-DDB1). Of note, two bands in cell lysates and immunoprecipitates were detected by using an anti-FLAG antibody. The lower band is the full-length AFAP-SF, based on the expected size (41.1 kDa). The upper band, whose size is approximately 50 kDa, is considered a modified form of AFAP-SF (mAFAP-SF).

    Article Snippet: A volume of 10 μL of the precipitates was probed using Western blot analysis with mouse monoclonal anti-FLAG antibody (cat#: M20008, Abmart, Shanghai, China), mouse monoclonal anti-NPM1 antibody (cat#: sc-32256, Santa Cruz Biotechnology), and rabbit polyclonal anti-DDB1 antibody (cat#: BS78489, Bioworld Technology).

    Techniques: Co-Immunoprecipitation Assay, Stable Transfection, Expressing, Immunoprecipitation, Modification